Mode of Transcription

IMPORTANT

Mode of Transcription: Overview

This topic covers concepts such as Transcription of DNA, Transcription Unit, Template Strand, Coding Strand, Post Transcriptional RNA Processing, Exons, Introns, Promoter sequence, Structural Gene, Terminator sequence, Monocistronic Gene, etc.

Important Questions on Mode of Transcription

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TATA box of eukaryotic promotor lies

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Origins of bacterial diversity through horizontal genetic transfer and adaptation to new ecological niches?

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What is the important role of HGT in eukaryotic genome evolution?

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Genes acquired by _____ have clear functional or ecological implications for their new host.

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HGT in eukaryotes has been overshadowed by its prevalence in bacterial genomes.

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Horizontal gene transfer (HGT), also known as? Explain about it.

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Which ONE of the following polymerases transcribes 5S rRNA?

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Which type of RNA polymerase enzyme transcribes precursor mRNA?

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Which type of RNA polymerase enzyme transcribes precursor mRNA? 

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In 1958 Francis _____ proposed that DNA determines the sequence of amino acids in a polypeptide through mRNA, this is the main principle or central dogma of protein synthesis. (Write the last part of the name)

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DNAX mRNA TranslationProtein

Identify the process"X" in the given flow chart.

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Find out the series of genes in the transcription unit of the following statements.

I. Located towards 3 '-end of the structural gene.

II.  Flanked by the promoter and the terminator.

III. Located towards 3'-end of the coding strand.

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Match the following

List-I List - II List -III
 A)  tailing  i) 28 s,18 s,5.8 s I) Transported out of nucleus
 B)  r-RNA  ii) Addition of methyl
guanosine triphosphate
II) 3 'end
C) Caping iii) m-RNA III) Transcribes by RNA polymerase
D) Processed
hn RNA
iv) Addition of Adenylate
Residue
IV) 5 ' end of hn RNA

The correct answer is

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Assertion (A) : During transcription both the strands of DNA are copied

Reason (R):   If both strands act as template DNA would code for RNA molecule with different sequences

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Assertion (A): The structural gene in a transcription unit can be said to be monocistronic or polycistronic.

Reason (R): In eukaryotes, the monocistronic structural gene has interrupted coding sequence - but the genes in eukaryotes split. The coding sequence or expressed sequences are defined as exons. Exons are said to be those sequence that appears in mature or processed RNA.
The correct answer is

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What is the corresponding sequence of mRNA when a polynucleotide template DNA strand is 3'-TAC GGT AAG CGG ATC-5'?

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Read the following statements and choose the correct option.
(i) Tailing: It is addition of adenylate residues at 5' -end in a template independent manner.
(ii) Promoter: It is the site recognised by the RNA polymerase in association with ρ factor.
(iii) Division of labour: RNA polymerase I and RNA polymerase III are responsible for 18S rRNA and tRNA transcription respectively.
(iv) Capping: addition of methyl guanosine triphosphate at the 5'-end of hnRNA.

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If the template strand of DNA is 3'ATGCATGCATGCATGC 5', identify the sequence of RNA transcribed.

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Find out the series of genes in the transcription unit of the following statements

(I) Located towards 3 ' end of the structural gene

(II) Flanked by promoter and terminator

(III) Located towards 3 ' end of the coding strand

HARD
IMPORTANT

The process of transcriptional termination is catalysed by